Anti-PEG antibody ELISA (mouse IgG specific) - EL-141-PEG-mIGG

Anti-PEG antibody ELISA (mouse IgG specific) Catalogue Number: EL-141-PEG-mIGG Size: 1X96 wells Antigen: Anti-PEG mouse IgG antibodies Storage: -20°C, 1 year Reactivity: Mouse Protocol: This assay employs the sandwich enzyme immunoassay technique. Immobilized PEG is coated onto a 96 well microplate. Calibrator and test samples are pipetted into the appropriate wells. Anti-PEG antibodies present in biological matrices is bound by the immobilized PEG. After washing away any unbound substances, enzyme linked anti IgG or IgM antibody is added to the wells. The plate is washed to remove any unbound antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of anti-PEG antibodies present in test samples. The color development is stopped and the intensity of the color is measured Components: Coated microtiter plate, 96 wells QC samples - 6x250ul 10X wash buffer - 50 ml Assay buffer - 50ml 1000X d