
Recombinant Leptin, Rat - BK0120
Recombinant Leptin, Rat Catalogue Numbers: BK0120-200, BK0120-1000 Sizes: 200μg, 1mg Source: Escherichia coli Molecular Weight: 16.3 kDa, observed by reducing SDS-PAGE. Purity: > 95% as analyzed by SDS-PAGE and HPLC. Biological Activity: ED50 < 10 µg/mL, measured by a cell proliferation assay using LoVo cells, corresponding to a specific activity of > 100 units/mg. Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder. Formulation: Lyophilized after extensive dialysis against 50 mM Tris, pH8.0. AA Sequence: MVPIHKVQD DTKTLIKTIV TRINDISHTQ SVSARQRVTG LDFIPGLHPI LSLSKMDQTL AVYQQILTSL PSQNVLQIAH DLENLRDLLH LLAFSKSCSL PQTRGLQKPE SLDGVLEASL YSTEVVALSR LQGSLQDILQ QLDLSPEC Endotoxin: < 0.2 EU/μg, determined by LAL method. Reconstitution: Reconstituted in ddH2O at 100 μg/mL. Storage: Lyophilized recombinant rat Leptin (rrLeptin) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rrLeptin remains stable up to 2 weeks