Recombinant VEGF164, Mouse (P.pastoris-expre Ssed) - BK0350

Recombinant VEGF164, Mouse (P.pastoris-expre Ssed) Catalogue Numbers: BK0350-20, BK0350-100 Sizes: 20μg, 100μg Source: P. pastoris Molecular Weight: 39kDa, observed by non-reducing SDS-PAGE. Purity: > 97% as analyzed by reducing SDS-PAGE Biological Activity: Resuspend cells in assay media (Earle Salts 199X Gibco #11150, without h-EGF) containing 10 % HI-FBS and transfer 100 ul/well to assay plate (5,100 cells/well passage#2). Serial dilute murine VEGF in assay media containing 10 % HI-FBS and transfer 100ml/well to cells in assay plate. Final assay volume is 200 ml/well, containing 10 % HI-FBS, and murine VEGF as indicated 89 hours. Add 20 ml of Promega Substrate Cell Titer 96 Aqueous One Solution Reagent to each well, Incubate 37°C and read at OD 490 nm. The ED50 for the effect is typically 4.1 - 6.2 ng/ml. Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder. Formulation: Lyophilized after extensive dialysis against 25 mM HEPES and 150 mM NaCl, pH