Algae Culture Kit

5 Gallon Algae Culture Kit All the basics(with exception of the tank) to grow your own useful strain of algae. Instructions: information about the strain of algae, illustrated growing protocol, culture care lessons, lifecycle background of batch-culture cycles. Culture flask: tissue culture flask (50 ml volume) with a breathable cap. Culture inoculum: 50ml in a dense culture in a 50ml centrifuge tube. Nutrient Fertilizer: our nutrients are a modification of the media formula called f/2 (Guillard and Ryther 1962, Guillard 1975). It uses NaNO3 (sodium nitrate) and NaPO4 (sodium phosphate) as the nitrogen and phosphorus sources. Culturing Salts: Add salts to a 5-gallons of chlorine free drinking water.