BDHC Substr. Kit (Cat#003850)

Benzidine Dihydrochloride (BDHC) Peroxidase Substrate Kit (Catalog Number: 003850) (Distributor and quantity discounts available, please send an email tocontact@bioenno.com to order. For common questions and answers, please check our FAQ.) Benzidine Dihydrochloride (BDHC) is a very sensitive chromogen and can produce a blue and particulate reaction product in the presence of peroxidase (HRP) enzyme under acidic conditions. The Bioenno BDHC Substrate Kit contains the reagents necessary to prepare an acidic buffer (pH 6.2 ± 0.1) (1,800 ml) and a BDHC substrate working solution (280 ml).  The kit is ideal for dual-labeling immunocytochemistry and immunohistochemistry and can be used together with 3,3’-diaminobenzidine (DAB), the most popularly used chromogen. The blue/particulate reaction product of BDHC is easily distinguished from the brown/diffuse reaction product of DAB (see images and references below). The kit has been tested on tissue sections and cells derived from mice and rats.