1-Step 2X Super Multiplex RT-PCR Master Mix-TaqMan Probe-Genetic Tools

Intended Use The 1-Step 2X Super Multiplex RT-PCR Master Mix is used for real-time qualitative and quantitative multiplex RT-PCR amplifications with TaqMan probes for up to four targets. The master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers, probes and RNA templates.   Product Characteristics The kit is designed for multiplex RT-PCR with TaqMan probes. For the reverse transcription step, this kit uses a highly efficient Thermophilic Reverse Transcriptase (US patent pending), which is a thermophilic type A polymerase, with optimal temperatures of 60-62°C. The RTase is easily heat-inactivated at ≥90°C for 1min. The RTase efficiently synthesizes complementary DNA strands on RNA templates from gene-specific primers, ≥1 unit per 20μL of reaction. The RTase reversely-transcribes single digit copies of target RNA molecules consistently. The kit also contains Super Taq-Probe DNA polymerase, specially engineered