Crystal Violet

Specification Grade Indicator Formula C25H30N3Cl MW 407.99 CAS 548-62-9 Appearance Dark green or dark brown crystals Absorption test PASS Non-aqueous titration PASS Ethanol dissolution test PASS Loss on drying ≤6.0% Residue on ignition ≤0.50% Organic impurities PASS Storage Store at room temperature. Protect from moisture.   Application Crystal violet is used as an active component, primary stain, of Gram stain for differentiation of Gram-negative versus Gram-positive bacteria.It has been used to check cell viability. It has also been used for the staining of cells to study cell migration and invasion. Biochem/physiol Actions Crystal violet can be used for DNA visualization in agarose gels. The dye is used only in the presence of high concentrations of DNA. Crystal violet is also used for the staining of bacteria in gram staining technique. It is also used for the staining of plant chromosomes.  Crystal Violet also helps in colorimetric measurement of cell v