dNTP mixture (25mM)

Product Description Product Summary  DNase, RNase: None detected. Suitable for use in the Polymerase Chain Reaction (PCR). dNTP Mix is a solution containing each of the four deoxynucleotides as follows: 25 mM dATP  25 mM dCTP 25 mM dGTP  25 mM dTTP   PCR Suitability dNTP Mix was tested at a final concentration of 200 μM in a reaction mixture containing10 mM Tris-HCl, pH 8.3 at 25 °C, 50 mM KCl, 1.5 mM MgCl2, 0.001% (w/v) gelatin, primers defining an approximately 500 base pair region of λ DNA at 1.0 μM each, λ DNA template at 1 ng/100 μl, and Taq DNA polymerase at 2.5 units/100 μl. The reaction underwent 25 cycles of 94 °C to denature the double stranded DNA, 55 °C to anneal the DNA segments, and 72 °C to extend the DNA segments. A single band of approximately 500 base pairs was visualized following electrophoresis of the reaction product in a 1.5% agarose gel. Endonuclease-Exonucleas One μg of λ Hind III fragments was incubated for 16 hours at 37 °C with dNTP