PhoX crosslinker for mass spectrometry

PhoX, also known as (3,5-bis(((2,5-dioxopyrrolidin-1-yl)oxy) carbonyl)phenyl)phosphonic acid It is a crosslinker used to study protein structures and interactions by means of Cross-Linking Mass Spectrometry (XL-MS). PhoX is a novel and unique trifunctional crosslinker. Two N-hydroxysuccinimide (NHS) ester functions react rapidly and irreversible with amino functions in proteins, mainly neighboring lysine groups. The stable phosphonic acid function allows for the enrichment of mono- and crosslinked peptides by using the well-established immobilized metal affinity chromatography (IMAC).  Overview of the available crosslinkers is shown in the table below. DSAU DSPU DSBU PhoX Type NHS ester Urea derivate NHS ester Urea derivate NHS ester Urea derivate NHS ester Phosphonic acid  Chain C2 C3 C4 Chain length ~7.7 Å ~10.1 Å ~12.5 Å ~ 5 Å   Examples of applications can be found in the following application notes and publications Improve your XL-MS results using t