CHAPS 10%, Sterile

CHAPS (3-cholamidopropyl dimethylammonio 1-propanesulfonate) is a non-denaturing zwitterionic detergent for solubilizing membrane proteins and breaking protein-protein interactions. CHAPS is commonly used for protein solubilization in isoelectric focusing and two-dimensional electrophoresis, especially for non-denaturing (without urea) isoelectric focusing. CHAPS is used at concentrations between 1-4% (v/v) that are generally used in an isoelectric focusing gel. A commonly used isoelectric focusing sample solution consists of 8 M urea, 4% CHAPS, 50mM DTT and 40 mM Tris. Its small micellar molecular weight (6150) allows it to be removed from samples by dialysis. bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.