
Cytek<sup>®</sup> cFluor<sup>®</sup> Human B Cell Monitoring Kit
The Cytek® cFluor® Human B Cell Monitoring Kit identifies B cell subsets in human peripheral blood mononuclear cells and in whole blood collected in EDTA, heparin, or CytoChex® BCT tubes. This 13-color cFluor B Cell Monitoring assay distinguishes plasmablasts/plasma cells (CD19+ CD20- CD27+ CD38++), naïve B cells (CD27- IgD+), IgG class switched memory B cells (CD27+ IgG+) and classifies unswitched memory B cells (CD27+ IgD+ and/or IgM+). Additionally, the kit identifies the T cell (CD3+) subsets of CD4+ and CD8+ T cells. The granulocyte and monocyte markers, CD15 and CD14, facilitate a cleaner lymphocyte gate. PRODUCT DETAILS Tested Dilution: 5 μL / test Application: Flow Cytometry Storage: 2-8°C and protected from Light. Do not freeze. Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (Origin USA) Data Sheets: TDS SDS EXAMPLE DATA Figure1: Gating strategy for identifying B cell subsets using the Cytek®