Muse^® Autophagy LC3-Antibody Based Kit

The Muse® Autophagy LC3-Antibody based kit provides a quantitative solution for studying autophagy. This kit contains two key detection reagents to facilitate the monitoring of lipidated LC3-II in a given cell system, and works by: Employing a selective permeabilization solution to discriminate cytosolic LC3 from autophagic LC3 by extracting the soluble cytosolic proteins while protecting LC3, which has been sequestered into the autophagosome Utilizing an autophagy detection reagent (Autophagy Reagent A) to prevent the lysosomal degradation of LC3, allowing for its quantification by flow cytometry The Muse Autophagy LC3-Antibody based kit includes an anti-LC3 mouse monoclonal antibody conjugated to Alexa Fluor® 555 to measure and track the levels of LC3 within the cell. The anti-LC3 Alexa Fluor® 555 conjugated antibody and autophagy enabling reagents have been carefully evaluated to ensure optimal performance, alleviating the need for any additional validation of the kit reagents. In