Muse^® PI3K/MAPK Dual Pathway Activation Kit

The Muse® PI3K/MAPK Dual Pathway Activation kit is designed to enable researchers to have a quick and easy way to cross-examine both the PI3K and MAPK signaling pathways simultaneously. Recent evidence suggests that there is cross-talk between the PI3K and the MAPK signaling pathways, therefore, antibodies for phosphorylated Akt (pAkt) and phosphorylated ERK (pERK) can be used to examine PI3K/MAPK interactions. Data generated using the Guava® Muse® cell analyzer provides statistical values, measuring the: Percentage of negative cells (e.g., no PI3K or MAPK pathway activation) Percentage of ERK1/2 activated cells (MAPK pathway activation) Percentage of Akt activated cells (PI3K pathway activation) Percentage of dual pathway activation (MAPK and PI3K pathway activation) The Muse PI3K/MAPK Dual Pathway Activation kit includes two directly conjugated antibodies-a phospho-specific Akt (Ser473)-Alexa Fluor® 555 and a phospho-specific ERK1/2 (Thr202/Tyr204, Thr185/Tyr187)-PECy5 conjugated a