Mouse IgG ELISA Kit

Product overview: Cytodiagnostics Mouse IgG ELISA kit is a sensitive and accurate assay for the quantitative determination of mouse IgG levels produced in vivo and for assessing the level of immunoglobulin secretion by a hybridoma in vitro. The Mouse IgG ELISA kit is based on the antibody sandwich principle. A microtiter plate coated with a capture antibody specific to mouse IgG Fc has been blocked and stabilized to create the solid phase of the assay. To perform the assay, samples, standards, and controls are added directly to the wells of the plate. After washing away unbound IgG, an HRP-conjugated Detection Antibody Solution is added and binds to the heavy and light chains of the captured mouse IgG protein that were immobilized by the capture antibody, completing the sandwich. The wells are washed and a tetramethylbenzidine (TMB) Substrate Solution is added. A blue colour develops in proportion to the amount of bound mouse IgG. The color development is stopped using Stop Solutio