RPMI-1640, With L-Glutamine and HEPES

RPMI-1640 was developed in 1966 by Moore and his co-workers at Roswell Park Memorial Institute (hence the acronym RPMI). It is based on the RMPI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. While it was originally formulated to support lymphoblastoid is suspension culture, it has been proven to support a wide variety of anchorage-dependant cells. It has a wide range of uses, including the culture of fresh human lymphocytes, fusion protocols, and in the growth of hybrid cells. With L-Glutamine With HEPES specification D-Glucose 2000 mg/L L-Glutamine 300 mg/L HEPES 5958 mg/L Phenol Red 5 mg/L   Store at 2-8℃