Stop Solution For TMB

TMB Stop Buffer (TSB) offers a unique combination of acid that produces a more stable stopped reaction product than other formulations of H2SO4 or HCl. Stopped reactions show increased absorbance values of approximately two-fold over unstopped reactions with less than 3% drift over six hours. Store at RT Peak Wavelengths 650 nm, unstopped, blue reaction product450 nm, stopped, yellow reaction product Reaction Temperature Room temperature Increased Absorbance Values Use of a stop solution enhances sensitivity 2-4 fold and the resulting yellow solution can be read at 450 nm.