Blot Stripping Buffer II 4X, pH 2.5

Each L of Blot Stripping Buffer II (4x), Low pH - contains enough liquid concentrate to make 4L of 1X solution. Diluted product (1X) is 0.2M glycine and 0.05% Tween 20, has a pH value of 2.5.After stripping, neutralize pH to 6.8 with Tris buffer. Might need to add 0.6% beta mercaptoethanol before use if desired. To use this product, place the membrane into stripping buffer for 30-45 minutes with gentle agitation. Buffer should be heated to approximately 50°C for incubation. Wash membrane 3 times with TBST or PBST before reprobing. Product is ultra pure grade.