Human Colony Stimulating Factor 2 Granulocyte Macrophage ELISA Kit

Human Colony Stimulating Factor 2 Granulocyte Macrophage ELISA Kit from Innovative Research is intended for the quantitative determination of Human Colony Stimulating Factor 2, Granulocyte Macrophage in biofluid samples, such as serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids. This is a sandwich ELISA in strip well format with reagents for up to 96 tests . GMCSF present in samples reacts with an anti-Human GMCSF capture antibody coated and dried on a microtiter plate. After appropriate washing steps, an anti-GMCSF detection antibody conjugated to biotin binds to the captured analyte. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active GMCSF in the samples. A standard calibration curve is prepared along with the samples to be measured using dil