Mouse Colony Stimulating Factor 3 Granulocyte ELISA Kit

Mouse Colony Stimulating Factor 3 Granulocyte ELISA Kit from Innovative Research is intended for the quantitative determination of Mouse Colony Stimulating Factor 3, Granulocyte in biofluid samples, such as serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids. This is a sandwich ELISA in strip well format with reagents for up to 96 tests . GCSF present in samples reacts with an anti-Mouse GCSF capture antibody coated and dried on a microtiter plate. After appropriate washing steps, an anti-GCSF detection antibody conjugated to biotin binds to the captured analyte. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active GCSF in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Mouse GCSF. Dete