Agilent HPLC Column 10 um Non-Porous 4.6 x 250 mm

The Agilent 5190-2455 HPLC Column is a specialized high-performance liquid chromatography (HPLC) column designed for biopharmaceutical applications, particularly for the analysis of charge variants of monoclonal antibodies (MAbs), proteins, and peptides. This column features a 10 µm non-porous particle size and has dimensions of 4.6 mm internal diameter by 250 mm in length. The core of this column is its Bio WCX (Weak Cation Exchange) stationary phase. This phase consists of a weak cation exchange layer chemically bonded to the hydrophilic polymer coating of the non-porous particles. Non-porous particles are highly advantageous in bioseparations because they minimize diffusion distances for large molecules, leading to exceptionally high efficiency and resolution, crucial for resolving complex charge variants. The non-porous nature ensures efficient mass transfer and high resolution separations, while the weak cation exchange functionality allows for charge-based separations of biomolec