EarlyTox Caspase-3/7-D NucView 488 Assay Kit

This kit enables enable detection of apoptosis in intact cell populations based on the measurement of caspase-3/7 activity using either a fluorescence microplate reader or a fluorescence imaging system. Caspase-3 and caspase-7 are proteases that are activated during the execution phase of apoptosis. NucView 488 Caspase-3 substrate (formulated in DMSO or phosphate-buffered saline (PBS), for EarlyTox Caspase-3/7-D NucView 488 Assay Kit and EarlyTox Caspase-3/7 NucView 488 Assay Kit, respectively) is used to detect caspase-3/7 activity within intact cells without interfering with caspase activity. The substrate consists of a fluorogenic DNA dye that is coupled to the caspase-3/7 DEVD recognition sequence. Initially non-fluorescent, the substrate permeates the plasma membrane and enters the cytoplasm. In apoptotic cells, caspase-3/7 cleaves the substrate, releasing a high-affinity DNA dye that migrates to the cell nucleus and stains DNA with bright green fluorescence, with excitation at 50