TAP Medium

TAP Medium Directions For 2 L Total To approximately 1600 mL of dH2O, add each of the components in the order specified while stirring continuously. Bring the total volume to 2 L with dH2O. For 1.5% agar medium add 30 grams of agar into the flask; do not mix. Cover and autoclave medium. Allow to cool and store at refrigerator temperature. For agar medium, dispense before agar solidifies. # Component Amount Stock Solution Concentration Final Concentration 1 Beijerinck's Solution 100 mL/2 L 2 Phosphate Buffer Stock Solution 17 mL/2 L 3 Hunter's Trace Stock Solution 2 mL/2 L 4 Tris Acetate Stock Solution 20 mL/2 L