Recombinant Human NOGGIN (rHuNOGGIN ) - PR1103

Recombinant Human NOGGIN (rHuNOGGIN ) Catalogue Numbers: PR1103-5, PR1103-20 Sizes: 5µg, 20µg Source: Escherichia coli Molecular Weight: Approximately 46.2 kDa non-disulfide-linked homodimer consisting of two 206 amino acid polypeptide chains. Purity: >95% by SDS-PAGE and HPLC analyses. Biological Activity: The ED50 was determined by its ability to inhibit 5.0 ng/ml of BMP-4 induced alkaline phosphatase production by ATDC-5 chondrogenic cells. The expected ED50 for this effect is 0.05-0.08 µg/ml of NOGGIN, corresponding to a Specific Activity of ＞1.25 x 104 IU/mg. Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder. Formulation: Lyophilized from a 0.2μm filtered concentrated solution in 30% acetonitrile, 0.1% TFA. AA Sequence: MQHYLHIRPAPSDNLPLVDLIEHPDPIFDPKEKDLNETLLRSLLGGHYDPGFMATSPPEDRPGGGGGAAGGAEDLAELDQLLRQRPSGAMPSEIKGLEFSEGLAQGKKQRLSKKLRRKLQMWLWSQTFCPVLYAWNDLGSFWPRYVKVGSCFSKRSCSVPEGMVCKPSKSVHLt VLRWRCQRRGGQRCG WIPIQYPIIS ECKCSC Endotoxin: Less