1-Step 2X RT-PCR Master Mix-TaqMan Probe-Genetic Tools

 Intended use The 1-Step 2X RT-PCR Master Mix is used for real-time qualitative and quantitative RT-PCR amplifications with TaqMan probe. The master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers, probe and RNA template.   Product Characteristics The kit is designed for singleplex RT-PCR with TaqMan probe. For the reverse transcription step, this kit uses a highly efficient Thermophilic Reverse Transcriptase (US patent pending), which is a thermophilic type A polymerase, with optimal temperatures of 60-62°C. The RTase is easily heat-inactivated at ≥90°C for 1min. The RTase efficiently synthesizes a complementary DNA strand on RNA template from a gene-specific primer, ≤1 unit per 20μL of reaction. The RTase reversely-transcribes single digit copies of target RNA molecules consistently. The kit also contains Taq-Probe DNA polymerase specially engineered for TaqMan probe, generating S-shaped curve. The concent