2X qPCR Master Mix-TaqMan Probe-Genetic Tools

Intended Use The 2X qPCR Master Mix is used for real-time qualitative and quantitative qPCR with TaqMan probe. The master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers, probe and DNA template.   Product Characterizations The kit is designed for singleplex qPCR with TaqMan probe. This kit uses Taq-Probe DNA polymerase specially engineered for TaqMan probe, which increased 5’ to 3’ exonuclease efficiency produces S-shaped curve. The concentrations of the primers and probe are variable depending on specific assays and thermocycling protocols (User Manual Table 1). The preferable PCR product size is ≤150bp. The kit has three formulations of ROX, Low ROX or High ROX concentrations for your choice (see User Manual Table 2).   Documents User Manual