Human Primary Brain Vascular Fibroblasts

Description Human Primary Brain Vascular Fibroblasts from Cell Biologics are isolated from human brain tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 2-3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x106 cells per ml and is delivered frozen. Human Primary Brain Vascular Fibroblasts are characterized by their spindle morphology and immunofluorescent staining with anti-fibroblast-specific protein 1 or anti-Vimentin antibody. These cells are negative for mycoplasma, bacteria, yeast, fungi, HIV-1, hepatitis B and hepatitis C and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended. Suggested Medium M2267 Complete Fibroblast Medium w/ Kit – 500 ml Storage Cryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrog