Human Primary Brain Vascular Smooth Muscle Cells

Description Human Primary Brain Vascular Smooth Muscle Cells from Cell Biologics are isolated from human brain tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 2-3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x106 cells per ml and is delivered frozen. Human Primary Brain Vascular Smooth Muscle Cells from Cell Biologics are characterized by immunofluorescence staining with antibody of α-smooth muscle actin. These cells are negative for mycoplasma, bacteria, yeast, fungi, HIV-1, hepatitis B and hepatitis C and can be expanded for 2-3 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended. Suggested Medium M2268 Complete Smooth Muscle Cell Medium w/ Kit – 500 ml Storage Cryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C)