
amfiSure qGreen Q-PCR Master Mix(2X), Without Rox
amfiSure qGreen Q-PCR Master Mix (2X) is a ready-to-use solution for quantitative real-time PCR and two-step real-time RT-PCR on most real-time PCR machines. The master mix includes amfiSure Hot-Start Taq DNA polymerase, dNTPs, qGreen Fluorescent DNA binding Dye (substitute for SYBR Green I dye) and rox passive dye in an optimized PCR buffer. amfiSure Hot-Start Taq DNA polymerase in combination Primers with an optimized buffer ensures PCR specificity and sensitivity. qGreen Fluorescent intercalating dye allows for DNA detection and analysis without using sequence-specific probes. dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG). The use of amfiSure qGreen Q-PCR Master Mix in real-time PCR ensure reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. amfiSure qGreen & qProbe Q-PCR Master Mixes contain heat-labile chemical blocker which enables standard Taq DNA Polymerase to b